广西防城港红树林根系土壤放线菌的分离和培养.pdfVIP

2009, 37( 12) : 130~ 131 Guizhou A gr icultural Science [ ] 2009) 1208550 13002 广西防城港红树林根系土壤放线菌的分离和培养 骆耐香, 陈森洲, 徐雅娟, 黄大林, 孔杰, 钟毓娟 ( , 541004) [ ] 为了解广西防城港海岸红树林根系土壤中放线菌的多样性, 从红树林根系土壤中分离出放线 菌后采 单菌落挑选与划线培养, 利 溶菌酶破壁蛋白酶K 和SDS 去除蛋白, 提取放线菌总DNA 结果: 从防城港海岸红树林根系土壤样品中分离出多株典型放线菌菌株, 并成功提取了总DNA 分离纯化获得多 株典型放线菌, 提取的总DNA 结构完整, 可为放线菌的进一步研究奠定基础 [ ] 红树林根系; 土壤; 放线菌; 防城港; 广西 [ ] Q933 [ ] A Isolation and Culture of Soil Actinomyces around Mangrove Roots in Fangchenggang City of Guangxi LU O Naix iang, CHEN Senzhou, XU Yajuan, H UANG Dalin, KONG Jie, ZH ONG Yujuan (D ep artment of Micr obiology I mmunology , Guilin Med ica l Univers ity , Guilin, Guangx i 541004, China) Abstract: T he election and cultur e of ingle colonie of actinomyce i olated from the oil of mangrov e ro ot and the total DNA of the colo nie w a ex tracted by u ing ly ozyme, pr otea e K and o dium dodecyl ulf ate ( SDS) to under tand it biodiver ity. The re ult how ed that om e typical train of actinomy ce w ere i olated fr om the oil of m angrove root and it total DNA w ith a complete tr ucture w a extracted ucce fully, w hich lay a foundation for further re ear ch of actinomyce . Key words: mangrove root ; oil; actinomyce ; Fang chengg ang ; Guangxi 2008 4 12 7: 00- 8: 00 , , , 1000 m , 15~ 20 cm , , 25 , 1. 1. 2 培养基 1 : 2% , , 1%KN O3 , 0. 05% NaCl, 0. 001% FeSO4 , 0. 05% , 8 000 M gSO4 , 2% , 0. 0 1% ( m/ m) , , 70% , , pH 7. 2~ 7. 4; ISP2 : 4. 0 g, [ 1] 10% 10. 0 g, 4. 0 g, 20. 0