一个水稻雄性不育突变体的遗传分析和基因定位.pdfVIP

作物学报 ACTA AGRONOMICA SINICA 2009, 35(6): 1151−1155 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2009.01151 一个水稻雄性不育突变体的遗传分析和基因定位 初明光 李双成** 王世全 邓其明 张 婧 丁 磊 文 勇 郑爱萍 周星宇 李 平* , 611130 : ms-np , , , , , , , , 5 F 2 BC F , 1 2 1 1 ms-np/M63 F2 , ms-np(t) 6 RM541 RM343 , 15.2 cM 7.9 cM : ; ; ; Genetic Analysis and Molecular Mapping of a Male Sterile Mutant in Rice ** CHU Ming-Guang, LI Shuang-Cheng , WANG Shi-Quan, DENG Qi-Ming, ZHANG Jing, DING Lei, * WEN Yong, ZHENG Ai-Ping, ZHOU Xing-Yu, and LI Ping Rice Research Institute, Sichuan Agricultural University, Wenjiang 611130, China Abstract: Male sterility is an important inheritance phenomenon in plants and widely used in hybrid seed production. The male sterile plant cant produce normal male gametophyte for reproduction, while the female counterpart is normal. So far, more than 100 of male sterile mutants or genes have been reported in rice. Ms-np is a male-sterile mutant derived from a spontaneous muta- tion. The filaments of the mutant are long and thin, and the withered anthers are white and transparent. Ms-np was confirmed to be a none-pollen type mutant of male sterility, for no pollen grains were stained with I2-KI solution and the anther locules were al- ways hollow. Genetic analysis of five F populations and two BC F populations revealed that the mutation was controlled by a