活血化痰方不同工艺提取物干预心梗大鼠心肌重塑与研究.pdfVIP

活血化痰方不同工艺提取物干预心梗大鼠心肌重塑的研究 郭丽丽 林飞 何永侠 王阶 (中国中医科学院 广安门医院. 北京100053) 摘要：目的 以瓜蒌薤白为基方，结合现代临床用药组成活血化痰方，研究该方水提和醇 提两种工艺提取物对心梗大鼠心功能及心脏结构的影响，探讨《金匮要略》瓜蒌薤白类 方佐伍白酒煎服的科学依据。方法 活血化痰方分别进行水提醇沉、60%醇提工艺进行提 取，提取物按人等效剂量饲喂心肌梗死大鼠，分别在心梗术后8 日和22 日两个时间点运用 超声技术检测大鼠心功能；术后23天取心脏组织，进行HE和Masson染色，分析心肌组织 病理变化。结果 饲喂60%醇提取物组大鼠在心梗术后8 日即出现阻止心梗后心脏EF、ES 值下降趋势，但与模型组比较不具统计学意义（P0.05) ，术后22 日时本组大鼠EF、FS 值下降程度明显降低，与模型组比较具有显著统计学意义（P0.01) ；其作用与减轻心肌 组织纤维化程度（P0.05) ，减少心肌坏死面积（P0.01)相关。水提醇沉物虽然能阻止EF 下降，降低心肌梗死面积、减轻心肌纤维化程度的作用趋势，但与模型组比较差异无统 计学意义（P0.05) 。结论 由瓜蒌薤白为基础的活血化痰方60%醇提物较之水提取物具有 明显干预心肌梗死后大鼠心脏恶性重塑，阻止心功能下降的作用，证明《金匮要略》中 瓜蒌薤白类方伍白酒煎服的科学意义，为传统方剂的现代应用奠定基础。 关键词：瓜蒌；薤白；活血化痰方；心肌梗死；提取工艺；60%醇提物 The Effect on Ischemic Myocardial Remodeling by Different Extract from HuoXue HuaTan Prescription ABSTRACT:OBJECTIVE Huoxuehuatan prescription consisted of Combined Trichosanthes kirilowii Maxim with Allii macrostemi bulbus were used as the basic medicines to reatment the syndrome of phlegm and stagnated blood of coronary heart diseases.To study the influence of the aqueous extract and alcohol extract from Huoxuehuatan prescription on rat myocardial infarction cardiac function and structure, explore the scientific basis of Trichosanthes kirilowii Maxim with Allii macrostemi bulbus matching baijiu decoction in Synopsis of Golden Chamber. METHODS Huoxuehuatan formula is extracted by water and ethanol precipitation or 60% alcohol,feeding myocardial infarction rats with the equivalent dose extract.detecting the cardiac function in rats by using ultrasound at 8 and 22 d .Taking heart for HE and Masson staining and then analysising the pathological changes of myocardial tissue at 23 d. RESULTS The rats which feed with 60% alcohol extract appear to prevent EF, ES value decline after 8th,but there is no significant difference compared with model group (p 0.05).Rats EF, FS value decline significantly lower