人子宫内膜基质细胞和腺上皮细胞的分离纯化和培养.pdfVIP

2007 10 31 10 867 人子宫内膜基质细胞和腺上皮细胞的分离 纯化和培养 (563003) 杨智敏 谭兵兵 王 丽 分离和纯化人子宫内膜基质细胞和腺上皮细胞并进行体外培养, 建立胚胎 体外种植模型奠定基础 通过酶消化筛网过滤和差时贴壁等方法, 分离和纯化人子宫内膜基 质细胞和腺上皮细胞并继续体外培养, 通过免疫细胞化学染色进行细胞表型鉴定 基质细胞和 腺上皮细胞的产量分别 8 106 个/ g 组织和6 106 个/ g 组织, 存活率分别 90% 和85% , 纯度分 别达95% 和90% ; 基质细胞波形蛋白染色阳性, 腺上皮细胞角蛋白染色阳性; 基质细胞接种后0. 5 h 开始贴壁, 腺上皮细胞在接种24 h 后贴壁; 基质细胞可以有限的传代, 腺上皮细胞不能传代 采用酶消化筛网过滤和差时贴壁等方法, 可获得纯度较高的子宫内膜基质细胞和腺上皮细胞人子 宫内膜细胞体外培养的成功 胚胎体外种植模型的建立奠定了基础 子宫内膜 基质细胞 腺上皮细胞 细胞培养 The culture of stromal and glandular epithelial cells of human endometrium Yang Zhimin, Tan B ing bing , Wang L i . Center of Rep r oductiv e Medicine , Af f iliated H o p ital of Zuny i M ed ical Col lege , Zuny i 563003 Abstract Objective To set up an invitro mo el of embryo implantation by separating, purificat ing an culturing stromal an glan ular epithelial cells of human en ometrium. Methods H uman en o metrial st romal an glan ular epithelial cells w ere separate an purificate by enzymolysis, mesh fil t ration an paste w all, then tw o types cells of purify w ere culture Phenotype of stromal an glan u lar epithelial cells w as i entif ie by immunohistochemistry . Results The yiel s of stromal an glan ular 6 6 epit helia cells were 8 10 / g an 6 10 / g respectively , the surviving rate of tw o kin s cells w ere 90% an 85% respectively. Stromal cells w ere positive for viment i. n, an glan ular epithelial cells positive for cytokeratin. Stromal cells became a herent after 0. 5 h of culture, glan ular cells became a herent after 24 h of cult ure. St romal cell coul be passage for several generations, but glan ular epit helial cells coul not be passage . Conclusion highly purifie st romal an glan ular epithelial cells of