农药影响植物甲基化与基因表达.pdf

Biotechnol Lett DOI 10.1007/s10529-015-1789-1 ORIGINAL RESEARCH PAPER Gene chip analysis of Arabidopsis thaliana genomic DNA methylation and gene expression in response to carbendazim Zhongai Li • Zicheng Wang • Suoping Li Received: 23 December 2014 / Accepted: 4 February 2015 Springer Science+Business Media Dordrecht 2015 Abstract to detect organic food and a new interpretation for the Objectives To examine the effects of carbendazim degradation of crop varieties. on Arabidopsis genomic DNA methylation and gene expression. Keywords Carbendazim DNA chip DNA Results Carbendazim caused widespread changes in methylation Epigenetics Farm chemicals Gene gene loci methylation and gene expression. With expression Microarray 0.1 mM (D2) and 0.2 mM (D3) carbendazim, there were, respectively, 1522 and 2278 demethylated sites and 1541 and 2790 methylated sites. A total of 279 and 505 genes were up-regulated by more than 300 % and Introduction 175 and 609 genes were down-regulated by 67 % in D2 and D3 treatments, respectively, compared with Pesticides are the main means of controlling plant the control. Conjoint analysis showed that 20 and 39 diseases and insect pests but are one of the risks in demethylated genes were up-regulated [300 % and 21 agriculture (Thompson 2010). The scatter and residues and 24 methylated genes were down-regulated \67 % from these pesticides can cause serious pollution in D2 and D3, respectively. (Ghimire and Woodward 2013) and great harm (Wang Conclusions Carbendazim causes methylation or et al. 2013), including many diseases to humans (Jaga demethylation of