各种刺激对家兔离体肠肌运动的影响.pdf

各种因素对家兔离体肠肌运动的影响 【摘要】目的：观察HCl、NaOH、低温、肾上腺素、乙酰胆碱、缺氧对肠道平滑肌收缩运动的影响并探究其原理和机制。方法：分别给予离体家兔 肠肌标本HCl、NaOH、低温、肾上腺素、乙酰胆碱、缺氧刺激，完成每次刺激并出现明显现象后用37℃～38 ℃台氏液冲洗肠肌标本，观察其收缩活动的 特点并记录。结果：HCl、低温和肾上腺素可减弱离体肠肌的舒缩强度，NaOH 和乙酰胆碱可增强离体肠肌的舒缩强度，缺氧对离体肠肌运动的影响因实 验误差较大并不明显。结论：HCl、低温和肾上腺素可抑制离体肠肌的运动，NaOH 和乙酰胆碱可增强离体肠肌的运动。 【关键词】小肠；肌收缩；盐酸；氢氧化钠；肾上腺素；乙酰胆碱 【Abstract 】Objective: HCl, NaOH, temperature, epinephrine, acetylcholine, hypoxia on contraction of intestinal smooth muscle and to explore its principles and mechanisms. Methods: Isolated rabbit intestinal muscles to give specimens of HCl, NaOH, temperature, epineph rine, acetylcholine, hypoxia stimulation, and the completion of each stimulus after the apparent phenomenon with 37 ℃ ~ 38 ℃ Tyrodes solution wash intestinal muscle were observed on The characteristics of its contractile activity and record. Results: HCl, temperature, and adrenaline can weaken the muscle relaxation and contraction of isolated intestinal strength, NaOH and acetylcholine can enhance the relaxation and contraction in isolated in testinal muscle strength, muscle hypoxia on isolated intestinal motility was not due to large experimental error Obvious. Conclusion: HCl, cold and adrenaline in vitro inhibit the movement of intestinal muscles, NaOH and acetylcholine can enhance the in vitro intestinal muscles. 【Key words】Intestine, Small; Muscle Contraction; Hydrochloric Acid; Sodium Hydroxide; Epinephrine; Acetylcholine 消化道平滑肌的舒缩活动与食物的机械性消化、化学性 动改变明显时，冲洗1 遍，再加入等量的37℃～38℃的台氏 消化以及吸收过程是密切相关的，细胞间的缝隙连接可使电 液。 信号在细胞间传递。消化道平滑肌具有肌肉组织的共同特性， 1.4.2.4 肠活动恢复后，向台氏液中加入1:10 000 肾上腺素 [1] 如兴奋性、传导性、和收缩性，同时又具有其他自身特点 。 2 滴，观察小肠活动的改变， 待反应稳定后冲洗2 遍，再加 入等量的37℃～38℃的台氏液。 1 材料和方法 1.4.2.5 肠活动恢复后，向台氏液中加入1:10 0000 乙酰胆 1.1 实验对象 家兔，浙江中医药大学动物实验中