拟南芥中热胁迫相关microRNA的差异表达 Differential Expression of Heat Stress Related microRNA in Arabidopsis thaliana.pdf

湖南农业科学2012，(03)：10—13 Hunan Sciences A咖cultural 拟南芥中热胁迫相关microRNA的差异表达 冯静弦，汪启明，胡 琪，饶力群 (湖南农业大学生物化学与分子生物学研究室，湖南长沙410128) 摘要：对改良CTAB法、改良热酚法和Trizol法3种方法在提取拟南芥叶片的总RNA中的效果进行了比较，并利用半定量 达情况都有差异，随着处理时间的延长，其表达量均出现明显提高的情况。 关键词：拟南芥；micmRNA；RNA提取；热胁迫；RT-PCR 中图分类号：Q943．2 文献标识码：A 文章编号：1006—060X(2012)03—0010-04 in thaliana Differential ofHeatStressRelatedmicroRNA Expression Arabidopsis FENG Qi-ming，HUQi，RAOLi-qun Jing-xian，WANG Research andMolecular DepartmentofBiochemistryBiology,HNA以Changsha41012PRa ofA extraction Abstract：Toestablishthe for totalRNAfromleavesrabidopsisthaliana,the optimalsystemextracting of methodandTrizol effectsthree modifiedCTAB method，were methods，whichincluding method，modifiedhot-phenol weredetected RT—PCR ofheatstressrelatedmiRNAs byusingsemi—quantitative compared，meanwhile，theexpressions ofA resultsshowedthat TrizolmethodCanextract RNA technology．The using highqualit)，total rabidopsisthaliana,and its effectwasbetterthanthatof CTABmethodandmodified of exⅡaefing modified hot-phenolmethod；theexpressions thefourscreenedmiRNAswereinducedheat the ofeach weretreatedat38℃ stress，but miRNA，which by