大孔吸附树脂分离纯化金钱草总黄酮工艺研究.pdf

HeraldofMedicineV01．28No．5 2009 ·636· May 大孑L吸附树脂分离纯化金钱草总黄酮工艺研究 陶锋1，李向荣2，占洁1 (1．浙江医学高等专科学校药学系，杭州310053；2．浙江大学城市学院药学系，杭州310015) [摘要】 目的研究大孔吸附树脂分离纯化金钱草总黄酮的最佳工艺条件及参数。方法 已各种浓度梯度的 乙醇洗脱后，以紫外分光光度法测定金钱草总黄酮收率和纯度为指标。结果 实验条件下，采用NKA-9型大孔树脂进 行吸附，6倍量纯化水冲洗，然后用50％乙醇50mL洗脱，经大孔树脂处理后得到总黄酮的纯度可达60．8％。结论该 法可为金钱草制剂的研制与生产提供原料药。 [关键词]金钱草；总黄酮；大孔树脂 【中图分类号]R284．2；TQ450．1[文献标识码]A IsolationandPurificationofTotalFlavonoidsformChr括tina Herbwith Loosestrife Resin Macroporous TAO Medical Fen91，LIXiang—ron92，ZHANJiel(1．DepartmentofPharmacy，ZhefiangCollege，Hangzhou 310053，China；2．DepartmentofPharmacy，CityCollege，ZhejiangUniversity，Hangzhou310015，China) ABSTRACT To the conditionfor totalflavonoidsfromChristina Herbwith Objective Loosestrife exploreoptimal purifying on resin．MethodsGradientelutionWaE,usedtoelutethe absorbted resinthe macroporous components macroporousby degree Results oftotalflavonoidsinobtainedointmentCOuldreach60．8％underthe of andrecoveryrate． The purification purlty conditionthatNKA-9of resinwas and50mL50％ethanolusedaselution typemacroporousused舾absorptionagent agent． be to for and oftotalflavonoidsfromChristina ConclusionThemethodCOuldused crudematerisis provide studyproduction Herb． Loosestrife KEYWORDSChristina resin Herb；Totalflavonoid