大花蕙兰花粉离体萌发试验初报.pdf

Chinese ScienceBulletinV01．21 No．42005 Agricultural April · 122- http：／／zntb．chinajournal．net．cn 大花蕙兰花粉离体萌发试验初报 王利民1一，王四清1，王彩霞2，刘慧2 (1北京林业大学园林学院，北京100083；z安阳工学院园艺园林系，河南安阳455000) 摘要：采用开花当天的大花蕙兰花粉，设计了多种人工花粉萌发培养基对其培养，结果证明大花蕙 兰的人工花粉萌发培养基的蔗糖浓度应为30％，添加50mg／L 芽。试验发现大花蕙兰的花粉在人工培养基上萌芽所需时间较长，约4d左右。萌芽后很难继续伸长， 可见其花粉的人工培养有一定的难度。 关键词：大花蕙兰；花粉人工萌发培养基；萌芽率 I helmitativePollen of BuddingExperimentationCymbidiumhybridium Hui2 Caixia2，Liu WangLiminL2,WangSiqingl,Wang A (1CollegeofLandscaperchitecture，Beiiing而阳st可University100083； 2Horticultureand Technical nYang landscapedop口tmem，A College，Henan455000) were for of Abstract：Inthis culturemediums pollen Cymbidium experiment，various designed budding saidthat ofsucrose result was and consistency 50mg／LGA3 hybridium．The appropriate 30％．Adding After tomedium the of Scatteredonartificial O．15％CaCl2 improvedbuddingCymbidiumhybridium． culture of a timefor medium，bloomingintradaypollenCymbidiumhybrdiumarequiredlong Budding．This 4 wasabout tubew踮hardto after indicatedthatthe process days