大鼠自体血栓结合线栓阻塞大脑中动脉制备脑缺血模型的建立与评价.pdfVIP

272 2006 5 18 5 Ch n Cr t Care M ed, M ay 2006, V ol. 18, N o . 5 李建生　刘敬霞　于海滨　王冬 ( M CA ) ( 10 ) ( 12 ) ( 12 ) M CA ( M CAO ) , , , , , , M CA M CA O 6 h , ; , , , ( P 0. 01) , ; , ( P 0 . 05) M CA , , M CA O ( M CA ) / , , ; ; ; Establishment and evaluation of reproduction of middle cerebral artery occlusion to produce cerebral - * , - , - , ischemia with autologous blood clot and nylon thread in rat L I Jian sheng L I U Jing x ia YU H ai bin WA N G Dong . * Dep artment of Geriatrics, H enan College of Tra itional Chinese Me icine, Zheng z hou 450003, H enan, China ( ) Abstract Obj ective T o r epro duce cerebra l schem a u s ng m ddle cer ebral art er y o cclu s on M CA O method w t h aut olog ou s blo od clot and nylo n thread n r at . Methods Rat s w er e r ando mly d v ded nt o sham -o per at o n gr oup ( n = 10) , suture-occlu s o n g ro up ( n= 12) , thr ombu s-sut ur e occlus o n m odel g ro up ( = 12) ( - ) . n n w h ch bot h auto lo go us blo od clot and nylon thr ead w ere u sed thr ombu s occluded g ro up Cerebr al schem a as a r esult of M CA O n suture-occlu s o n g ro up w as r epl cated by nser t o n o f ny lo n t hr ead . In thr ombu s-occluded g r oup , a poly ethylene cat het er co nt a n ng a nylon thr ead w as nser ted nto the m ddle caro t d arter y v a ext ernal car ot d ar ter y, and the nylon thread w as pulled out to an appr opr ate leng th t o allo w bloo d t o coag ulat e. F fteen m nutes later , thr omb n w as njected , and the nylon thread w as r e-nsert ed to complete M CA O . Spec mens w er e taken aft er schem a fo r 6 hours