骨碎补提取物对骨髓间充质干细胞向成骨细胞分化及其CXCL12表达的影响及其意义.pdfVIP

骨碎补提取物对骨髓间充质干细胞向成骨细胞 分化及CXCL12表达的影响及意义 刘国岩1徐展望1徐琬梨2 1山东中医药大学附属医院，济南250014 2山东中医药大学，济南250355 摘要：目的：体外实验研究中药骨碎补对大鼠骨髓间充质干细胞(BMSCs)的增殖及分化，及对趋化因子 CXCL12分泌的影响机制。方法：采用中药干预培养细胞的方法，利用骨碎补单味药物与培养基联合培养大鼠 BMSCs，通过ALP染色检测其促BMSCs成骨分化能力、RT-PCR检测其促BMSCs分泌趋化因子CXCLi2能力。结果： 骨碎补药物各组ALP染色检测BMSCs成骨分化能力均明显优于空白组及条件培养组 （P＜0.05），骨碎补药物各组 之间没有显著性差异。骨碎补药物各组RT-PCR检测CXCL12的表达明显优于空白组及条件培养组 （P＜0.01）， 0.002g/mL药物组优于0.02g/mL及0.0002g/mL药物组(P＜0.05)。结论：中药骨碎补可促进BMSCs成骨分化和增 殖，促进BMSCs分泌趋化因子CXCL12。 关键词：骨碎补；骨髓间充质干细胞；趋化因子CXCL12 EffectsandsignificanceofdrynariaextractonexpressionsofCXCL12andosteogenic differentiationofbonemarrowstromalcells LIUGuo-yan1XUZhan-wang1XUWan-li2 1AfflliatedHospital,ShandongUniversityofTraditionalChineseMedicine,Jinan250014,China 2ShandongUniversityof TraditionalChineseMedicine,Jinan250355,China Abstract:Objective:Tostudytheeffectofdrynariaextractonproliferationanddifferentiationofratbonemarrow stromalcells(BMSCs)andthemechanismofpromotingthesecretionofthechemokineCXCL12invitro.Methods:Withthe methodofinterferenceofChinesemedicinecellculture,singledrynariacombinedwithmediumco-cultureofratBMSCs,inspect abilityofosteogenicdifferentiationofBMSCsthroughalkalinephosphatasestaindetectionandabilityofincreasingthesecretion ofCXCL12throughRT-PCRdetection.Results:Thealkalinephosphatasestainoftestingindexesthatthedrynariadruggroups weresuperiortoblankgroupandconditionalgroup(P＜0.05),thedrynariadruggroupshadnoobviousdifferences.Theresults ofCXCL12expressionbyRT-PCRdetectionshowedthedrynariadruggroupsweremuchbetterthantheblankgroupandthe conditionalgroup(P＜0.01),the0.002g/mLdrynariagroupwassuperiorto0.02g/mLand0.0002g/mLdrynariagroups(P＜0.05). Conclusion:TraditionalChinesemedicinedrynariacouldpromoteosteogenicdifferentiationofBMSCsandincreasethesecretion ofchemokineCXCL12. Keywords:Drynaria;B