4.2.11AOACOfficialMethod2001.11Protein(Crude)inAnimalFeed美国公职分析化学师协会标准AOAC.pdfVIP

4.2.11 tion procedures must be used in order to include N from nitrate.) The AOAC Official Method 2001.11 amount of protein in most materials is calculated by multiplying % N Protein (Crude) in Animal Feed, by 6.25, because most proteins contain 16% N. Forage (Plant Tissue), Grain, and Oilseeds The H2SO4 and NaOH used are in concentrated form and are Block Digestion Method Using Copper Catalyst highly corrosive. Wear gloves and eye protection while handling the and Steam Distillation into Boric Acid chemicals. Do not mix concentrated acid and NaOH directly. If First Action 2001 chemicals are splashed on the skin or in the eyes, flush with copious [Applicable to the determination of 0.5–50% Kjeldahl N amounts of water. Seek medical attention. Do not breathe the sulfur (3–300% equivalent crude protein) in forage, animal feed and pet oxide fumes produced during digestion. food, grain, and oilseeds, and applicable to the same matrixes as B. Apparatus 976.05 (4.2.05), 976.06 (4.2.06), 984.13 (4.2.09), 988.05 (4.2.03), (a) Digestion block .—Aluminum alloy block with adjustable and 990.02 (4.2.07); the method does not measure oxidized forms of temperature device for measuring and controlling block temperature N or heterocyclic N compounds.] (Tecator Digestion System 20, 1015 Digestor, Foss North America, See Tables 2001.11A and B for the results of the interlaboratory 7682