HSP70_肿瘤肽对小鼠肝癌的抗肿瘤免疫效应.pdfVIP

2002 S p; 9( 3) : 163~ 167 Chin J Canc r Bioth r # 163 # [ ] 1007-385X( 2002) 03-0163-05 HSP70- 1 2 3 陈代雄 , 苏艳蓉 , 钱振超 ( 1. 遵义医学院附属医院贵州省细胞工程重点实验室, 遵义 563003; 2. 北京大 学医学 免疫学系; 3. 大连医科大学肿瘤研究所生物治疗中心) [ ] : HSP70- MHC- Ñ: , HCaF HSP70- HCaF( MHC- Ñ) : HCaF HSP70- , HCaF , HSP70- HCaF , , HCaF , 1@ 7 10 HSP70- , HCaF : HCaF HSP70- MHC- Ñ, , [ ] 70- ; ; [ ] R730. 51 [ ] A TheEffect ofAntitumorImmunityElicitedbyHeatShockProtein70-Associated Peptide Complexes on Hepatomain ice 1 2 3 CHEN Dai-xiong , SU Yan-rong , QIAN Zhen-chao ( 1. Key aboratory of Cell Engineering of Guizhou Province, The Aff iliated Hospital fo Zunyi Medical College; Zunyi, 563003, China; 2. Dep artment of Immunology, Health Science Center, Peking University , Beji ing 100083, China; 3. Research Center of Cancer Biotherapy , Institute of Cancer Research, Dalian Medical University, Dalian 116027, China) [ Abstract] Objective: To obs rv ff ctiv of antitumor immunity licit d by h at shock prot in70(HSP70)-associat d p ptid compl x s isolat d from h patoma (HCaF) , atumor mod l of no MHC- Ñmol cul xpr ssion in mic . ethods: Sp cific activ immunization and adoptiv c llular immunization assay was adopt d to obs rv th immunoprot ctiv ff ct licit d by HSP70-as- sociat d p ptid compl x s purfi d from th HCaF. Results: Mic immuniz d with HSP70-associat d p ptid compl x s w r prot ct d from HCaF chall ng . Th immunoprot ctiv ff ct in f mal mic was b tt r than th mal groups. Adoptiv ly trans- f rr d immun spl n c lls of mic which had b n immuniz d with HSP70-associat d p ptid compl x s could b licit d immu- nity against HCaF chall ng , and th mic fr of tumor can r sist r p at d chall ng , th maximal chall ng-quantiti s xc