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Comparison of Overexpression of Cbfa1 and BMP-2 on
Osteoblast/Cementoblast Related Genes Expression in
NIH3T3 and Dental Follicle Cells
Pan Keqing 1, Yan Shiguo 1‡,Ge Shaohua 1, Li Shu 1, Zhao Yanhong 2, Yang Pishan 1*
1 Department of Periodontology, School of Dentistry, Shandong University, Jinan (250012 )
2 Department of Orthodontics, School of Dentistry, Shandong University, Jinan (250012 )
E-mail :yangps@
Abstract
Objectives: BMP-2, one of the most widely studied factors has shown some shortcomings as a
therapeutic agent to enhance periodontal regeneration, while Cbfa1 is the essential transcription factor
for bone formation and tooth morphogenesis. The aim of this study was to compare the function of
Cbfa1 and BMP-2 on osteoblast/cementoblast related genes expression, so as to find which was more
useful for periodontal regeneration. Materials and Methods: Cbfa1 and BMP-2 recombinant retroviral
plasmids were transfected into packaging cells PT67 to produce retrovirus. Then the virus was added to
infect NIH3T3 and dental follicle cells (DFCs) and chosen by G418 to gain the stably overexpressed
Cbfa1 or BMP-2 cells. Real-time RT-PCR, western-blotting and mineralization assay were adopted to
determine and compare the effect of overexpression of Cbfa1 and BMP-2 in NIH3T3 and DFCs.
Results: We successfully obtained the cells that stably overexpressed Cbfa1 or BMP-2 by retrovirus
infection. In NIH3T3, both Cbfa1 and BMP-2 induced osteoblast/cementoblast related genes
expression and mineral nodules formation, but Cbfa1 increased more.
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