Compement.ppt

细胞膜上的调节因子 膜辅蛋白：MCP 抑制MAC形成： 衰变加速因子（DAF） C8结合蛋白 CD59 (膜反应性溶血抑制剂） 四、补体的生物学作用 溶菌杀菌细胞毒作用：MAC 免疫调理作用：C3b，C4b 免疫复合物清除：C3b，C4b 炎症介质： 过敏毒素 (C3a C4a C5a) 趋化作用 (C3a C5a C567) 激肽样活性 (C2a C4a) Opsonization and phagocytosis 思考题 细胞培养所用的血清为什么需要 56度30分钟灭活？ * * * * * * * * Classical Pathway Generation of C3-convertase C4b Mg++ C4a Ca++ C1r C1s C1q C2 C2a b C2 b C4b2b is C3 convertase Classical Pathway Generation of C5-convertase C4b Mg++ C4a Ca++ C1r C1s C1q C2a C2 b C3 C3a b C4b2b3b is C5 convertase; it leads into the Membrane Attack Pathway C4b C2b C3b C5-convertase of the Classical and lectin Pathways Components of the lytic pathway C6 C 9 C8 C7 C5 Lytic pathwayC5-activation C3b C2 b C4b C5 b C5a Lytic pathwayassembly of the lytic complex C5 b C6 C7 Lytic pathway:insertion of lytic complex into cell membrane C5 b C6 C7 C8 C 9 C 9 C 9 C 9 C 9 C 9 C 9 C 9 C 9 C3 转化酶形成 C3 正反馈循环 C5 转化酶形成 攻膜复合体形成 补体激活的旁路途径 Components of thealternative pathway C3 B D P 旁路途径 C3 C3b C3bB B D Ba C3bBb P(备解素） C3bBb3b C3转化酶 C5 转化酶 Spontaneous C3 activation C3 H2O i B D C3 C3a b Generation of C3 convertase This C3b molecule has a very short half life b Components of thealternative pathway C3 B D P B D b C3b If spontaneously-generated C3b is not degraded C3-activationthe amplification loop C3 C3a b C3a B D Bb C3b C3 b C3-activationthe amplification loop C3b C3a b C3a C3a Bb C3b C3b C3 Bb B D b b C3a C3-activationthe amplification loop C3b C3a C3a Bb C3b Bb Bb C3b C3a C3-activationthe amplification loop C3b C3b C3a C3a Bb C3b Bb Bb C3a C3-activationthe amplification loop C3b C3b C3b stabilization andC5 activation C3b C3b finds an activator (protector) membrane C3 C3a b C5 b C5a B D b P This leads to membrane attack pathway C3b Bb C3b C5-convertase of the Alternative Pathway Lytic pathway:insertion of lytic complex