Nisin与山楂提取物复配的抑菌性能研究.pdfVIP

,Journal of nhui gri. Sci.2009, 37( 15) :6839- 6840 常俊香 卢瑶 Nisin * 吕晓楠, 吴兆亮 , 赵艳丽, 殷 昊 (, 300130) [ 目的] 为开发新型天然防腐剂提供依据[ 方法] 以山 为材料, 枯草芽孢杆菌酿酒酵母和大肠杆菌为供试菌种,采用传统水煎 法提取山 中的有效成分,并向102030405060708090100l 提取物中分别加入10mg Nisin 制成复配物, 测定复配物对3种供试 菌的抑菌效果[结果]Nisin 的效价为994.5IU/ mg, 当山 提取物加入量为50l 时,效价达到1531 IU/ mg, 为Nisin 的1.53倍,但提取物 加入量超过50l 时,效价下降复配物对3种供试菌的抑菌效果好于单独使用山 提取物和Nisin[结论] 山 提取物与Nisin 的复配 物对3种供试菌均有较好的抑制效果, 且Nisin 与山 提取物的最佳复配比例为15(mg:l) Nisin; 山 ;抑菌性 TS202.3 0517- 6611(2009) 15- 06839- 02 Study on Antimicrobial Activity of Nisin Combined with Hawkthorn Extracts LV Xiaonan et al (Department of Bioengineering, HebeiUniversity ofTechnology, Tianjin 300130) Abstract [Objective] The aim was to provide the basis for developing new typesof naturalpreservative. [Method] With hawkthorn as thematerial and Bacillus Subtilis, Saccharomycescerevisiae andEscherichia colias thetestedstrains, the effective components inhawkthornwere extractedwithtraditional decoction method. nd then adding 10mg Nisin into 10, 20, 30, 40, 50, 60, 70, 80, 90, 100lextracts resp. to prepare the compound of nisin and hawkthorn extracts. The antimicrobialactivities of different compounds on 3 kinds of testedstrainswere measured. [Result] The titer of Nisinwas994.5 IU/mg. When50l hawkthorn extracts was added, the titer reached 1531IU/ mg, being 1.53times of that of Nisin. But when addition amount of the extracts was more than50l, the titer decreased. Theantimicrobial activitiesof compoundson 3kinds oftested strainswere better thanthose of single use of hawkthorn extractsandNisin. [Conclusion] The compounds of hawkthornextracts andNisin hadgood antimicrobial activity on 3kindsof tested strains and the optimal compound proportion of Nisinand hawkthorn extractswas 15(mgl) . Key words Nisin;Hawkthorn; ntimicrobial activity (nisin) ( Lactococcus . 2.2