植物乳杆菌发酵培养基的优化及其高密度培养技术.pdf

262 2011, Vol. 32, No. 07 食品科学 ※生物工程 植物乳杆菌发酵培养基的优化及其 高密度培养技术 熊 涛，黄锦卿，宋苏华，关倩倩，谢明勇 (南昌大学食品科学与技术国家重点实验室，江西南昌 330047) 摘 要：应用响应面法优化植物乳杆菌培养基配方以及利用中和法与指数流加法优化植物乳杆菌高密度培养的发酵 条件。在单因素试验基础上，进一步采用SA S 软件进行中心组合设计和响应面法优化发酵培养基。优化后的培养 基配方为：葡萄糖质量分数5 .4 3% 、蛋白胨质量分数0 .9 8% 、K2HP O4 质量分数0 .5 9% 。利用15L 全自动发酵罐， 在接种量3 % 、p H 6 .5 、培养温度3 5 ℃的最佳条件下，采用氨水中和发酵培养基和指数流加碳、氮源，最终发酵 9 液中植物乳杆菌菌体浓度达到9.3 ×10 CFU/mL 。 关键词：植物乳杆菌；响应面；培养基优化；高密度培养；指数流加 Lactobacillus p lantarum: Optimization of Fermentation Medium and Investigation of High-density Culture Methods XIONG Tao ，HUANG Jin-qing ，SONG Su-hu a ，GUAN Qian-qian ，XIE Min g-yong (State Key Laboratory of Food Science and Technology , Nanchang University, Nanchang 330047, China) Abstract ：In this study , respon se surface methodology was applied to optimize medium composition for L actobacillus p lantarum, and high-density cultivation of L. p lantarum was optimized by neutralization and exponential feeding method. On the basis of single-factor experiments, the optimal fermentation medium formula was determined by central composite design combined with response surface methodology to be made up of 5.43% glucose, 0.98% peptone and 0.59% K2HPO4 . Ammonia neutralization and exponential feeding carbon and nitrogen sources together operated in a 15 L fermenter under the optimal conditions (inoculation amount of 3%, pH 6.5 and cultivation temperature of 35 ℃) resulted in a final cell concentration of up to 9.3 ×109 CFU/mL. Key words ：L . p lantarum ；respon se surface ；medium optimization ；high-den sity culture ；exponential feeding batch 中图分类号：TS20 1.3 文献标识码：A