丹参注射液对肝性脑病的的防治作用及机制研究.pdf

中国药师　 　 2014年第17卷第11期　 　 China Pharmacist2014,Vol.17　 No.11 丹参注射液对肝性脑病的的防治作用及机制研究 1 2 2 1 胡瑜 　 蔡梦婷 　 陈健文　 陈浩凡 　 (1.广东省边防总队医院　 广东深圳518029;2. 中山大学药学院) 摘　 要　 目的:探讨丹参注射液对肝性脑病(hepatic encephalopathy,HE)的防治作用及其作用的初步机制。 方法:通过急性 氨中毒动物模型和四氯化碳(CCl )致肝损伤合并硫代乙酰胺(TAA)诱导髙血氨的大鼠肝性脑病模型,对氨中毒死亡时相、血 4 氨、脑氨水平、肝脏功能以及肝脏病理组织学改变等指标进行了评价。 结果:在急性氨中毒实验中,丹参注射液能使急性氨中 毒小鼠存活时间明显延长;在CCL 和 TAA诱导的肝性脑病大鼠模型中,丹参注射液能明显降低肝性脑病大鼠血氨和脑氨水 4 平,并可在一定程度上降低肝性脑病大鼠血清ALT、AST、ALP、总胆红素(Tbil)含量,改善肝功能,从而防治肝性脑病。 结论: 丹参注射液对肝性脑病有较好的防治作用,可能与降低血氨和脑氨水平有关。 关键词　 丹参注射液;肝性脑病;高血氨;硫代乙酰胺 中图分类号:R285.5　 　 文献标识码:A　 　 文章编号:1008-049X(2014)11-1811-05 Preventive and Therapeutic Effects and Underlying Mechanisms of Danshen Injections in the Treatment of Hepatic Encephalopathy 1 2 2 1 Hu Yu ,CaiMengting ,ChenJianwen ,ChenHaofan (1. GuangdongGeneralTeamHospitalofArmedPolice,GuangdongShenzhen 518029,China;2. School of Pharmaceutical Sciences,Sun Yat-sen University) ABSTRACT　 Objective:Toassessthepreventiveandtherapeuticeffectsandprimary mechanismsof Dansheninjectionsinthetreat- ment of hepaticencephalopathy (HE). Methods:Theacuteammoniapoisoningmodelinducedby ammoniumchloridewasestablished in mice,and HE modelwithliverinjury inducedby carbontetrachlorieandhighbloodammoniainducedbythioacetamidewasmadein rats. Thetimeof deathwasdetected inthefirst model,andthehistaminelevelsinblood andbrain,liverfunction andliver pathologi- cal histology changeswere detected respectively in the other model. Results:In the acute ammonia poisoning experiment,thetime of death in Danshen injections group was longer than that in the control group. Furthermore,Danshen injections could notably decrease the histamine levels in blood and brain,reduce the levels of ALT,AST,ALP and Tbil in serum and improve the function of liver to show thepreventiveandtherapeuticeffectsonHE.Conclusion:Dansheninjectionsexhibitpromisingpreventivea