大庆湖藻的分离、鉴定和高油脂含量筛选.pdf

62 生物技术 2013年23(1) llL日3rosis factor infectionofhuman fromBrucellamelitensisinhumanandanimalinfectionssmooth alphaproduction Omp31 by during macrophages and Lab [J]．InfectImmun．，2001，69(8)：4823—4830． roushBrucellae[J]．CfinOtagn to 115． [9]CassataroJ．，PasquevichK，Bruno【．，eta1．Antibodyreactivity 大庆湖藻的分离、鉴定和高油脂含量筛选 朱万鹏1，刘玉2，刘宇峰2，姬妍茹2，王莹莹1，石杰2，周广麒1 摘要：目的：对大庆6个典型湖泊的湖藻进行分离和鉴定，筛选出高油脂含量的大庆湖藻。方法：采用吸管分离法、稀释平板 分离法和平板划线法共分离出8株硅藻和5株绿藻，并利用化学方法对所分离的湖藻进行油脂含量测定。结果：所分离13株藻 含量的大庆湖藻。 关键词：大庆湖藻；分离；鉴定；油脂含量测定 中图分类号：Q938．8；Q547文献标识码：A doi：10．3969／j．issn．1004—311X．2013．01．017 IsolationandIdentificationofLake Algae in and OilContentsSelection DaqingHigh ZHU Yu2，LIU Yan—ru2，WANG Jie2，ZHOU Wan—pen91，LIUYu—fen92，JI Ying—yin91，SHIGuang—qil of Branchof of (1．SchoolBioengineering，DalianPolytechnicUniversity，Dalian HeilongiiangAcademySciences，Daqing 116034，China；2．Daqing andidentificationoflake insix lakesand oilcontents Abstract：Objective：Theseparation algae Daqingrepresentativehigh diatomsandfive straw methodandstreak meth— selection．Method：Separateeight greenalgaesby separationmethod，dilution—plate plate and areGF—l Cosmarium od．Result：Thethirteenof oilcontents Scenedesmus 9．3％。GF一2 2．7％，GF一3 types