对蓟马类害虫高致病性球孢白僵菌的分离、鉴定.pdf

茶叶科学201I，31(4)：295—299 Tea JournalScience of 对蓟马类害虫高致病性球孢白僵菌的分离、鉴定 王俊平，郑长英’ 青岛农业大学农学与植物保护学院，山东青岛266109 摘要：从草履蚧僵虫体内分离出12株病原真菌，对蓟马类昆虫具有70％～90％的致死率。现选出一株病原真菌 CYTl0利用形态和分子的特点进行鉴定。扫描电镜下观察，该菌产孢细胞簇生瓶状，“之”型产孢轴。经PCR 株与球孢白僵菌Beauveria bassiana聚为一类。根据菌株的形态观察和DNA序列分析确定该菌为球孢白僵菌。 关键词：球孢白僵菌：蓟马：形态特点；rDNA．ITS，EFl．a 中图分类号：$435．711 文献标识码：A of IsolationandIdentification highlypathogenic 1一 ■ - ■ ■ ·，1n’ ● BeaUverta17asslana1 againsthrips WANG Jun．ping，ZHENGChang．ying’ andPlant 266109，China CollegeofAgronomyProtection，QingdaoAgriculturaluniversity。Qingdao Abstract：12different isolatedfromdiseasedDrosicha Kuwanashowed fungi entomopathogenic corpulenta high virulencewith strainwasidentifiedbasedonthe andmolecular 70％～90％mortality．CYTl0 morphological cellswere to andthe ina characteristics．Conidiogenousshort—globoseflask-shapedconidiophore long elongated extensionaftereachconidiumis resultofBlastsearchindicatedthattheITSandEFI—a zig—zag produced．The a withthose ofCYTl0showed ofotherstrainofBeauveria sequence highsimilarity showedit relatedtoARSEF一1185 was to 10 thatwas B．bassiana．CYTstrain analysis closely strain，whichbelonging wasclassifieda o