Development of StabilityIndicating Methods for Cefquinome Sulphate.pdf

InternatIonal journal of B IomedIcal scIence ORIGINAL ARTICLE Development of Stability-Indicating Methods for Cefquinome Sulphate Shaza W. Shantier, Elrasheed A. Gadkariem, Mohamed O. Adam, Magdi A. Mohamed Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Khartoum, Sudan AbstrAct the degradation behavior of cefquinome sulphate in alkaline medium at different temperatures was investigated using both first derivative spectrophotometric and HPLC methods. The drug degradation was found to be pH and temperature dependant. The pH-rate profile indicated a first order dependence of Kobs on -] at pHs ranging between 9 and 11. Arrhenius plot obtained at pH 10 was linear between 65° and 100°C. [OH The estimated activation energy of the hydrolysis was found to be 21.1 kcal mol-1. Stability-indicating thin- layer chromatographic method for the separation of the drug and its alkaline hydrolysis product has been developed. (Int J Biomed Sci 2013; 9 (3): 162-167) Keywords: cefquinome sulphate; derivative spectrophotometry; HPLC; kinetics; suspension INtrODUctION Cefquinome Sulphate (CS) is Quinolinium (Fig. 1) (2): 1-[[(6R,7R)-7-[[(2Z)-(2-amino-4-thiazolyl)(methoxyimi- The need to develop a stability-indicating method us- no)acetyl]amino]-2-carboxy-8-oxo-5-thia-1-azabicyc- ing stress degradation has been recommended by Interna- lo[4.2.0]oct-2-en-3-yl]methyl]-5,6,7,8-tetrahydro-,sulfate. tional Conference of Harmonization (1). In practice, the CS has a broad spectrum of activity against both Gram- effe