Effect of substrate nitrogen on lignin degradation by Pleurotus ostreatus.pdf

Archives of Arch Microbiol (1985) 142:61 -65 Microbiology 9 Springer-Vertag1985 Effect of substrate nitrogen on lignin degradation by Pleurotus ostreatus Frank Commanday* and Joan M. Macy Department of Animal Science, University of California, Davis, CA 95616, USA Abstract. In order to determine the effect of substrate Kirk 1981), ligninolytic activity is stimulated by nutrient nitrogen (N) on ligninolytic activity, Pleurotus ostreatus was limitation; nitrogen (N)-limitation being the most effective grown in solid media containing either growth-limiting means of promoting longterm lignin degradation by (1 raM) or excess (10raM) NH4C1. After 25 days, 14C-CO2 Phanerochaete chrysosporium (Reid 1983 a, b, 1979; Buswell production from14C-cornstover lignin in low-N medium was et al. 1982; Kirk et al. 1978; Fenn et al. 1981). 3 times that in nitrogen (N)-rich medium. Supplementation In this study we examined the effect of growth-limiting of low-N medium with glucose (0.3%) further enhanced and excess substrate N (as NH4C1) on ligninolytic activity ligninolytic activity. Decolorization of an aromatic, in Pleurotus ostreatus. We also developed a novel method polymeric dye, Poly R-481, in solid media was also greatest for the preparation of l~C-cornstover lignin by (U-14C)- under N-limiting conditions. cinnamic ac