以L-谷氨酰胺-铜(Ⅱ)配合物为供体酶法制备茶氨酸.pdf

第59卷第12期 化 工 学 报 V01．59No．12 ofChemical and (China) December2008 2008年12月 Journal IndustryEngineering 以L一谷氨酰胺一铜(Ⅱ)配合物为 供体酶法制备茶氨酸 邓海霞，姚 忠，周 治，吴明刚，荀志金，徐 虹，韦 萍 (南京工业大学制药与生命科学学院，江苏南京210009) 关键词：茶氨酸；卜谷氨酰转肽酶；酶法合成；o谷氨酰胺一铜(Ⅱ)配合物 中图分类号：Q814．9 文献标识码：A ofL—theanine Enzymaticsynthesis using utamine asdonorsubstrate copper(Ⅱ)一L—gl complexes DENG Haixia。YAoZhong。ZHoUZhi，WUMinggang，XUNZhijin。XUHong。WEIPing (Sc^oDZ SciP以fP口，ld D，Li，i P^口r，，I口fy，№可f柙gLkiw，苫i￡yo，T-c_}lnoZDgy，j忆巧i”g210009，Ji口挖gs越，既i，l口) Anovelmethodfor ofL—theanine (Ⅱ) Abstract： enzymaticsynthesis usingcopper 一L—glutamine donorsubstratewas structureof wasidentifiedinfrared [Cu(G1n)2]as proposed．The Cu(Gln)2 by its wasalso underthereactionconditions．The and spectrumanalysis stability investigated enzymatic ofL—theanine wascarriedout andL广 synthesis catalyzedby7LglutamyltranspeptidasebyusingCu(Gln)2 asdonorsubstrate andthe andconversionrateofdonorsubstrate yield 91utamine respectively，product theconditionsofdifferentratiosofdonorand resultsshowedthatthe werecomparedunder acceptor．The be asdonor reactioncould enhanced transpeptidation effectively byusingCu(Gln)2 conversionrateofdonorwouldincrease 44．9％and27．1％underdifferentreactionconditions，