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Available online at Comparative Biochemistry and Physiology, Part C 147 (2008) 30 – 35 /locate/cbpc Dynamic changes in parameters of redox balance after mild heat stress in aged laying hens (Gallus gallus domesticus) H. Lin a,b , D. De Vos c , E. Decuypere b , J. Buyse b, ⁎ a Department of Animal Science, Shandong Agricultural University, Shandong 271018, PR China b Lab of Physiology and Immunology of Domestic Animals, Department of Biosystems, Catholic University Leuven, Kasteelpark Arenberg 30, 3001 Leuven, Belgium c Centre for Surface Chemistry and Catalysis, Catholic University Leuven, Kasteelpark Arenberg 23, 3001 Leuven, Belgium Received 4 June 2007; received in revised form 19 July 2007; accepted 20 July 2007 Available online 1 August 2007 Abstract In order to evaluate the metabolic responses of laying hens induced by high temperature at later laying stage, nine 60-wk-old laying hens (Gallus gallus domesticus) were employed in the present study. The hens were exposed to 32 °C for 21 d and blood samples were obtained before and at 1, 7, 14 and 21 d of heat exposure. The reactive oxygen species (ROS) formed in blood during heat exposure were estimated by the ex vivo spin-trapping method. Body temperature and plasma concentrations of glucose, urate, creatine kinase (CK), triiodothyronine (T ), thyroxine (T ), 3 4 corticosterone (CORT), thiobarbituric acid reacting