IHNV单克隆抗体的制备及其初步应用.pdf

39 2 Vol. 39, No.2 2 0 1 5 3 ACTA HYDROBIOLOGICA SINICA Mar., 2 0 1 5 doi: 10.7541/2015.56 IHNV 1 2 1 1 1 1 曹 欢 景宏丽 王 姝 王静波 王小亮 徐立蒲 (1. , 100021; 2. , 100029) PREPARATION OF MONOCLONAL ANTIBODIES AGAINST IHNV AND ITS PRELIMINARY APPLICATION 1 2 1 1 1 1 CAO Huan , JING Hong-Li , WANG Shu , WANG Jing-Bo , WANG Xiao-Liang and XU Li-Pu (1. Beijing Fisheries Technical Extension Station, Beijing 100021, China; 2. Research Center of Aquatic Animal Diseases, Institute of Animal Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100029, China) : ; ; ELISA Key words: Infectious haematopoietic necrosis virus; Monoclonal antibody; Double antibodies sandwich ELISA 中图分类号: S941.4 文献标识码: A 文章编号: 1000-3207(2015)02-0426-05 (Infectious haematopoietic ne- crosis, IHN) , BALB/c , RPMI 1640 95%[1, 2], IHN HYCLONE , , (OIE), HATHT(PEG1500) GIBCO , , (HRP) , Ig(TMB) , [3, 4] SIGMA , IgG (Infectious Southern Biotech , CKX41 haematopoietic necrosis virus, IHNV), OLYMPUS , SANYO (Rhabdoviridae), (Novirhabdovirus) ; Avanti J-30I BECKMAN IHNV , PCR [5, 6], IHNV ,