MWNTsAChE新型生物传感器的制备及其在氧化乐果快速检测中的应用.pdf

6 2013,VoL34,No．04 良晶科学 ※工艺技术 of and ofaNovelBiosensorfor Detection DevelopmentApplication Rapid Dimethoate GUANHua—nanl．一，CHIDe—fu2一，YUJia2 ofFood of Commerce，Harbin150076，China： (1．CollegeEngineering，HarbinUniversity ofLife 1 Sciences，Northeast 50040，China) 2．College ForestryUniversity,Harbin Abstract：Anovel biosensorfor basedon films amperometric detectingorganophosphatepesticides multilayerprepared from carbon chitosan，multi—wallnanotubes(MWNTs)and bylayer—by—layerself-assembly was structureofMWNTs／AChEfilmswereobserved electron developed．The multilayer byscanning microscopy(SEM)， and ofthe biosensorsweremeasuredelectrochemicalworkstationandasa biosensor propertiesresulting using result，the basedonfive of filmswasfoundtobethebestone．A linear betweentheinhibitionof layersmultilayer good relationship AChE andelectriccurrentwhenthisbiosensorwasusedtodetectdimethoateintheconcentration dimethoate againstactivity ofO．25to1．50 and1．75to10．00 limitofdetectionwas10nmol／Lata ratioof3． ranges I_tmol／L I．tmol／L．The signal—to-noise TheThe biosensorexhibited and developed goodreproducibilityacceptable