“和血生络法”对PI3KAkt信号转导通路的影响.pdf

箧地塑笠：!塑查生垡鎏：：盟里望望叁堕信呈整昱适路笪髭喧筮!塑 “和血生络法”对P13K／Akt信号转导通路的影响 侯仙明 王亚利 方敬 贾云芳师旭亮 (河北医科大学，河北 石家庄050091) [摘要]目的通过研究“和血生络方”对心肌梗死模型大鼠左室心肌组织AKTmRNA表达的影响，从信号转导通路角度探讨“和血生络 法”促血管新生的作用机制。方法选雄性Wistar大鼠，制作心肌梗死大鼠模型，随机分为消心痛组，和血生络高、低剂量组及模型组，同时建立假手 术组，每组6只。药物治疗组分别给予和血生络水溶液(9．9，19．8g·kg～·d-1)及消心痛水溶液(2．7 mg·kg～·d。1)每天灌胃1次，其他组给予 等量蒸馏水，连续用药3W后处死大鼠，取心脏组织检测AKT 优于消心痛组(P0．05)。结论P13K／Akt信号通路在“和血生络法”促血管新生中起着重要作用。 [关键词]和血生络法；心肌梗死；P13K／Akt；bFGF [中图分类号]R285．5[文献标识码]A EffectsofHexue methodonPDK／Akttransduction Shengluo signal pathway HOU FANG a1． Xian-Mi雌，WANGYa-Lij Jing，et of TraditionalChineseMedicineHebeiMedical College 050091，Hebei，China University，shijiazhuang Toobservethe ofAKTmRNAinleftventricular tissueinratsanddiscuss 【Abstract】Objective expression myocardial angiogenesis mechanismofHexue methodfromthe transduction ofinfarctedratsmodelwere promoting Shengluo signal pathways．MethodsMyocardium madeWistar weredivided into andD insham weremadeatthesalne by rats．They randomlymodel，Y，Ggroups．Ratsgroup time．Every had6ratsandratswere after24hours．Ratsin treatmentwereadministratedwithIDwatersolutioninaccordance group lavaged drug groups with2．7 withHexue watersolutioninaccordancewith9．9and19．8 inmodelandsham mg·kg一1·d一‘and Shengluo g·kg一‘·d一1．Rats weretreatedwithdistilledwaterwiththe volume．Ratsweretreatedonce administration．After3weeks groups equal everydaybyintragastric wereexecutedandheartswere ofAKTmRNAofcardiactissuewasdetected．ResultsThe of continuously，rats got．Expression