心肌梗死论文基底膜基质胶携带人脂肪间充质干细胞移植改善心肌梗死大鼠心脏功能.docVIP

心肌梗死论文：基底膜基质胶携带人脂肪间充质干细胞移植改善心肌梗死大鼠心脏功能 【中文摘要】心脏是体内再生能力最低的器官之一,因此,心肌损伤到梗塞形成或其他疾病通常会导致心脏衰竭。干细胞为修复损伤的器官如心脏及其组成部分提供可能,而且已经有相当多的基于干细胞策略修复心肌的研究。由人脂肪组织来源间充质干细胞(adipose-derived mesenchymal stem cells, ADMSCs)分化而来的心肌细胞能为修复梗塞心脏提供大量潜在的细胞。然而,这一方法却被ADMSCs向心肌细胞的无效分化、制备的心肌细胞纯度不足及移植后心肌细胞低成活率所限制。细胞外基质(extracellular matrixc, ECM)是细胞赖以生存的微环境,是组织和器官的重要组成部分,在生物生长发育过程中有重要的结构和生物学作用。基底膜基质胶(basement membrane matrix, matrigel)是一种从EHS(engelbreth holm swarm)小鼠肉瘤中提取的细胞外基质复合物,其内含有层连蛋白、Ⅰ型胶原、类肝素、蛋白多糖、生长因子、基质金属蛋白酶等多种物质,可在细胞增殖、分化、迁移等过程中起重要作用。本研究应用natrigel携带人ADMSCs在大鼠心肌梗死部位注射,观察其对细胞存活的影响及改善大鼠心梗模型心脏功能的能力。本研究在无菌条件下分离、培养、扩增人ADMSCs.应用左冠状动脉前降支结扎法建立大鼠急性心肌梗死模型,将大鼠急性心肌梗死模型随机分为4组,对照组(PBS组)、Matrigel组、PBS+ADMSCs组、Matrigel+ADMSCs组,对移植4周后心梗局部新生血管的密度进行测量,并利用心脏超声检测大鼠心脏功能。结果显示,与其他各组相比,Matrigel+ADMSCs组心肌梗死区域新生血管密度显著提高,心脏超声结果表明Matrigel+ADMSCs组大鼠的心脏功能改善最为显著。本研究结果发现,单纯Matrigel注射便能够促进心肌梗死部位血管生成,Matrigel携带ADMSCs能显著提高梗死区域的微血管密度并改善心脏功能。Matrigel是一种良好的干细胞载体,在心肌梗死的细胞移植治疗中具有潜在的应用前景。 【英文摘要】The heart is one of the least regenerative organs in the body and, consequently, loss of myocardium to infarction or other diseases often leads to heart failure[1-4]. Stem cells offer the possibility of repairing damaged organs like the heart from their component parts, and there is an intensive effort to develop stem cell-based strategies for cardiac repair. Cardiomyocytes derived from human adipose-derived mesenchymal stem cells (ADMSCs) potentially offer large numbers of cells to facilitate repair of the infarcted heart. However, this approach has been limited by inefficient differentiation of ADMSCs into cardiomyocytes, insufficient purity of cardiomyocyte preparations and poor survival of ADMSCs derived myocytes after transplantation.Extracellular matrix (ECM), which plays important structural and biological roles in growth and develoment, is the microenvironment for cells to survive. It is also the essential component element of tissues and organs. Basement membrane matrix is a kind of extracellular matrix complex extracted from the EHS