phosphopeptide机理探讨.pdfVIP

ANALYTICAL BIOCHEMISTRY Analytical Biochemistry 323 (2003) 94–102 /locate/yabio Susceptibility of the hydroxyl groups in serine and threonine to b-elimination/Michael addition under commonly used moderately high-temperature conditions Wei Li,a Peter S. Backlund,b Robert A. Boykins,c Guiyu Wang,a and Hao-Chia Chena,* a Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA b Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA c Laboratory of Biophysics, Division of Bacterial, Parasitic, and Allergenic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA Received 11 June 2003 Abstract The b-elimination/Michael addition reaction has been employed for the modification of O-acylated and phosphorylated Ser and Thr residues in a variety of derivatives. The modified Ser and Thr can be analyzed by amino acid composition analysis, N-terminal Edman degradation sequence analysis, and tandem mass spectrometric sequencing which generally allows the identification and localiz