《A Label-Free Immunoassay Based Upon Localized Surface Plasmon Resonance of Gold Nanorods》.pdfVIP

A R A Label-Free Immunoassay Based Upon T Localized Surface Plasmon Resonance of I Gold Nanorods Kathryn M. Mayer,† Seunghyun Lee,‡ Hongwei Liao,‡ Betty C. Rostro,† Amaris Fuentes,† Peter T. Scully,† C Colleen L. Nehl,† and Jason H. Hafner†,‡,* † ‡ L Department of Physics Astronomy and Department of Chemistry, Rice University, Houston, Texas 77005 E old and silver nanoparticles ex- hibit localized surface plasmon ABSTRACT Robust gold nanorod substrates were fabricated for refractive index sensing based on localized Gresonances (LSPR) at visible and surface plasmon resonance (LSPR). The substrate sensitivity was 170 nm/RIU with a figure of merit of 1.3. To near-infrared frequencies, leading to sharp monitor biomolecular interactions, the nanorod surfaces were covered with a self-assembled monolayer and peaks in their spectral extinction.1 The de- conjugated to antibodies by carbodiimide cross-linking. Interactions with a specific secondary antibody were pendence of the resonance condition on the local dielectric environment enables a monitored through shifts in the LSPR spectral extinction pe