化学通报邻菲咯啉荧光猝灭法测定粉丝中的铝.pdfVIP

化学通报 2005 年 第68 卷 w061 邻菲咯啉荧光猝灭法测定粉丝中的铝 乔善宝 费承英 (盐城师范学院化学系 江苏盐城 224002) 摘 要 基于铝对邻菲罗啉的荧光具有猝灭的特性，拟定了一种测定痕量铝的新方法。该方法的 激发波长为235nm，发射波长为366nm 。线性范围为10µg ·L-1～1.0mg ·L-1 ，相关系数为0.9996，检 测限为0.96µg ·L-1 ，对于50µg ·L-1 的铝标准溶液测定10 次的相对标准偏差为 1.10%，用该方法测定 了粉丝中的微量铝，结果令人满意。 关键词 荧光猝灭 邻菲罗啉 铝 粉丝 Determination of Aluminium in Vermicelli Based on the Fluorescence Quenching of Phenanthroline Qiao Shanbao, Fei Chengying (Department of Chemistry, Yancheng Teachers’ College, Jiangsu Yancheng 224002) Abstract A new selective and sensitive spectrofluorimetric method for the determination of Aluminium in vermicelli has been developed. This method is based on the quenching of fluorescene of phenanthroline due to the formation of complex Al( Ⅲ) Phenanthroline. The excitation and emission wavelengths are found to be 235 and 366nm, respectively. The detection limit is 0.96 µg ·L-1 for Al. The RSD is 1.10% (n=10) for 50µg ·L-1Al. There is a linear relationship in the range of 10µg ·L-1～1.0mg ·L-1 for Al. This method has been applied to the determination of Aluminium in vermicelli with satisfactory results. Key words Fluorescence quenching, Phenanthroline, Aluminium, Vermicelli 人们一度认为铝是无害元素，现在这种观点已被摒弃。研究结果表明，铝是弱神经毒微量元 [1] [2] 素 。铝的生态学效应及其对人类健康的作用是近年来引人注目的一个课题 。虽然铝本身不具 备毒性，正常人每天的摄入量可达10～100mg。但铝可以在生物体内富集，达到一定浓度后就会 [3] 抑制生物体对钙的吸收和积累，导致生物体严重缺钙，进而引起体内一系列相关功能酶失活 。 另外，铝可使脑内去甲肾上腺素、多巴胺和5-羟色胺的含量明显降低，并使神经递质传导阻滞。