《linker-mediated recombinational subcloning of large DNA fragments using yeast》.pdf

Downloaded from on September 13, 2012 - Published by Cold Spring Harbor Laboratory Press Linker-Mediated Recombinational Subcloning of Large DNA Fragments Using Yeast Christopher K. Raymond, Elizabeth H. Sims and Maynard V. Olson Genome Res. 2002 12: 190-197 Access the most recent version at doi: 10.1101/gr.205201 References This article cites 37 articles, 15 of which can be accessed free at: /content/12/1/190.full.html#ref-list-1 Article cited in: /content/12/1/190.full.html#related-urls Creative This article is distributed exclusively by Cold Spring Harbor Laboratory Press Commons for the first six months after the full-issue publication date (see License /site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported License), as described at /licenses/by-nc/3.0/. Email alerting Receive free email alerts when new articles cite this article - sign up in the box at the service top right corner of the article or click here To subscribe to Genome Research go to: /subscriptions © 2002, Published by Cold Spring Harbor Laboratory Press Downloaded from on September 13, 2012 - Published by Cold Spring Harbor Laboratory Press Methods Linker-Mediated Recombinational Subcloning of Large DNA Fragments Using Yeast Christopher K. Raymond,1 Elizabeth H. Sims, and Maynard V. Olson The University of Washington Genome Center, Department of Medicine, University of Washington, Seattle, Washington 98115, USA The homologous recombination pathway in yeast is an ideal tool for the sequence-specific assembly of plasmids. Complementar