《三维同步偏振荧光光谱研究蛋白质溶液变性时氨基酸残基的发光行为》.pdf

2004 62 Vol. 62, 2004 12 , 1153~ 1157 ACTA CHIMICA SINICA No. 12, 1153~ 1157 , a a a a b 唐 波 杜 鸣 陈蓁蓁 张 慧 沈含熙 a ( 250014) b ( 300071) (TDSPS) (Trp) (Tyr) , .TDSPS( BSA) (HSA) () Trp, Tyr , HSA BSA. , ,, , Studies on Luminescence of Trp and Tyr Residues in Protein Denaturation by Three-dimensiona-l synchronous-polarized Spectrofluorimetry , a a a a b TANG, Bo D , Ming CHEN, Zhen-Zhen ZHANG, Hui SHEN, Han-Xi a ( College of Chemistry, Engineering and Materials Science, Shandong Normal University, Jinan 25001 ) b ( Department of Chemistry, Nankai University, Tianj in 300071) Abstract Based on polarizing, synchronous and three-dimensional technologies, a new three-dimensiona-l synchronous-polarized spectrofluorimetry (TDSPS) was used to distinguish tryptophan (Trp) and tyrosine (Tyr) residues in albumin solution with the advantages of abundant information of three-dimensional technology and high resolution of synchronous spectrofluorimetry. In this paper, thefluorescent spectral changes of Tyr andTrp residues in bovine serum albumin ( BSA) and human serum albumin ( HSA) were studied by obtaining the characteristic info