不同来源黄芩的红外光谱鉴别.docVIP

不同来源黄芩的红外光谱鉴别 摘要 目前对黄芩含量的研究取得了一定的成果，但关于不同部位及不同来源的黄芩质量的影响还没有相关文献报道。本文采用分辨率高、准确度好, 简便快捷、无污染的FTIR光谱法对不同部位及不同来源的红外光谱图进行比较，研究了河北黄芩的FTIR光谱特征。黄芩共有峰为11处,其峰的数目、形状及相对强度可用于区分黄芩的不同部位、不同来源。结果表明：黄芩各药材与黄芩苷相比，黄芩苷有26个吸收峰，黄芩药材有12到16个吸收峰，其中，在600～1800 cm-1之间的指纹区中各吸收峰比较尖锐，峰宽比较小，而药材中各吸收满天飞的吸收峰形状比较圆钝，峰宽较大等情况，这可能是药材中化合物比较复杂，相近物质比较多，相邻吸收峰相互叠加所致，但从不同黄芩部份及不同来源的吸收峰的差异来比较各药材的区别。黄芩木材与黄花皮比较可得，黄花皮的在596、686、767、1069、1245、1359、1415、1450、1614、2927、3387 cm-1附近各吸收峰的相对强度均大于黄芩木材的。黄芩栽培品与野生品比较可得，黄芩野生品在以上各吸收峰相对强度均大于栽培品。而黄芩根类药材与黄芩叶的光谱图比较可得，相同或相近的吸收峰较少。这些吸收峰相对吸收强度的改变可能某些成分的减小有关，这已经有相关的研究。但不同的吸收光谱的特证性吸收峰有助于快速鉴别不同部位、不同来源黄芩质量的影响变化，所以红外光谱是一种快速临鉴定药材的方法。因此, 可以利用FTIR指纹图谱方便快捷地区分黄芩的不同部位、不同来源。 关键词　FTIR光谱法;黄芩; 吸收峰 Using FTIR spectroscop to idengtify different geographical origins of scullcaps Abstract At present the studies of scullcaps’s content has made some achievements, but there is no relevant reports about the influence of different parts and different sources of scullcaps in the literature. This paper adopts FTIR, which is high resoluted , good accurate, simple, rapid and pollution-free, to compare the radix scutellariae of the scullaps from different parts and different sources and then studies the FTIR spectra characteristics of Hebei’s scullcaps. Scrllcaps have 11 peaks ,whose peak number, shape and relative strength can be used to distinguish the scullcaps from different parts and different sources. The results show that baicalin scutellariaes have 26 absorption peaks, while radix scutellariae medicinal materials have 12 to 16 absorption peaks.The absorption peak in 600 ~ 1800 cm - 1 of the baicalin scutellariaes is comparatively sharp and the peak width is comparatively small, while the radix scutellariae medicinal materials’ absorption peak is quite obtuse and the peak width is bigger. The reason is that the compound of radix scutellariae medicinal materials is more complex, similar substance is more, near absorption peaks caused stack with each other. We can idengtify the different parts of scullcaps