《A UPLC-DAD–MS Method for the Quality Analysis of ‘JiangYaBiFeng’Tablet》.pdf

Latin American Journal of Pharmacy Regular Article (formerly Acta Farmacéutica Bonaerense) Received: January 4, 2012 Revised version: February 1, 2012 Lat. Am. J. Pharm. 31 (1): 125-32 (2012) Accepted: February 5, 2012 A UPLC-DAD–MS Method for the Quality Analysis of ‘JiangYaBiFeng’ Tablet Lin ZHU, Siwei PENG, Olajide OLALEYE, Aidi QI, Xin CHAI, Haitao LIU Yuefei WANG * Tianjin State Key Laboratory of Modern Chinese Medicine and Tianjin Key Laboratory of TCM Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, 300193 Tianjin, PR China. SUMMARY. ‘JiangYaBiFeng’ (JYBF) tablet for treatment of hypertension in China is a composite pre- scription of Chinese and western medicines. By using ultra high performance liquid chromatography cou- pled with mass spectrometry (UPLC-MS), twenty-five compounds were simultaneously identified or tenta- tively characterized based on their retention times and MS spectra. Nine target compounds, hy- drochlorothiazide (HC), rutin, genistin, sophoricoside, baicalin, wogonoside, genistein, baicalein and wogonin, were further quantified by ultra high performance liquid chromatography with diode-array de- tector (UPLC-DAD). Chromatographic separation was successfully performed on a C18 column with gra- dient elution of 0.1 % formic acid aqueous solution and acetonitrile at the flow rate of 0.4 mL/min in 15 min at 52 °C. Different wavelengths were used to determine corresponding compounds to ensure the best resolution. According to the methodological validati