Revised Supplementarydata.doc

Table S1. Primer sets used for the initial exons sequencing of the IL23R gene Exon Exon Length (bp) PCR primers (sense/antisense) PCR Products Length (bp) Annealing Temperature Exon1 56 ATAGTGACACGAGAGCCAGTCATTT 577 65℃ CAATGCATGGCTCATCATATCGCAT Exon2 99 AACTCAGAGGAGGAGGTTTCTGATG 648 63℃ AGGGCTGTCTAGAAGGGAAATTTGA Exon3 297 AGATACATCTAGTCCTAAAACTGT 838 55℃ ACAAGAAGATTAAAAGAGCAACA Exon4 124 GGTGTGCCATTTGCTGAGACTGT 634 55℃ GTAGGAAATAAGGGATAAATCAGGGTA Exon5 161 AGGCACATGCCACCAATTATTGT 539 59℃ GCAGCACTACTCATCTCTAAGTTGTC Exon6 146 AGGATTGTAAGGGAGTGAAAGGTG 506 63℃ AAGTCCACTGTAGCCACTGATGGTT Exon7 157 CATCTCAAAAAAAGCAGTGTGTGTT 632 63℃ GCTCACGCAATCCTACTACCTCA Exon8 90 GCCTCATGAAATTAGTGGACACACC 563 65℃ GCAAAATCCACCTAAGCCACTTCTT Exon9 103 GCACTTCTAAAAACCCACTGACATC 633 65℃ AGTGATTCTGGGCTGAGGACTTAG Exon10 91 TGGCATGGTCACAGCTCACTACA 624 63℃ CCCCCAACTCTATTGTCTCATCTCT Exon11-1＊ 1502 TGGAGGGAGAAAGGAAAGTTGAAAG 641 63℃ AAGCAGGGTCTGTTCTGGAATAGTT Exon11-2＊ 1502 TCCCAGGTTACAAAAGCATCCTAAT 676 63℃ TTCCACCTTCGGGACCTTAATTCTC Exon11-3＊ 1502 GTGACATTTCTGTGCTCCTACCAT 745 63℃ CACTTATTTTGTCATTTGGGGCTAT Exon11 has 1502 bps, so the fragment containing exon11 is too long to be sequenced directly by ABI 3730 Genetic Analyzer. Therefore, this fragment was sliced into 3 shorter fragments. The middle short one repeated partial sequences of the other short fragments at both sides. These 3 short fragments were then amplified using a pair of primers, respectively. Table S2. Thirteen SNPs identified in IL-23R gene by exons resequencing from ten pairs of pulmonary TB cases and controls dbSNP ID PositionGRCh37.p13 Region Variant Genotype Case control rs1884444Exon2 G/T GG 2(0.2) 0(0.0) GT 4(0.4) 8(0.8) TT 4(0.4) 2(0.2) r67633963 Intron2 C/T CC 8(0.8) 10(1.0) CT 2(0.2) 0(0.0) r67635475 Intron3 C/T CC 5(0.5) 2(0.2) CT 4(0.4) 8(0.8) TT 1(0.1) 0(0.0) r67648294 Intr