人脐带间充质干细胞联合番茄红素对衰老小鼠免疫功能影响.docVIP

人脐带间充质干细胞联合番茄红素对衰老小鼠免疫功能的影响 刘? 佳1，王意忠1，崔晓兰2，时? 瀚1，李倩倩1，王琳琳1 1北京大学航天临床医学院血液内分泌科，北京市? 100049；2中国中医科学院中药所药理室，北京市? 100700 Effects of human umbilical cord mesenchymal stem cells combined with lycopene on immune function of D-galactose induced aging mice Liu Jia1, Wang Yi-zhong1, Cui Xiao-lan2, Shi Han1, Li Qian-qian1, Wang Lin-lin1 1Department of Hematology and Endocrinology, Aerospace Clinical Medical College of Peking University, Beijing 100049, China; 2Pharmacology Laboratory, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China 摘要? 背景：人脐带间充质干细胞、番茄红素均有延缓衰老的作用，而二者联合应用对衰老机体免疫功能影响的研究却未见报道。目的：分析人脐带间充质干细胞联合番茄红素对D-半乳糖诱导衰老小鼠免疫功能的影响。方法：ICR小鼠雌雄各半，随机分为正常对照组、模型组、番茄红素组、干细胞组及干细胞联合番茄红素组。模型组与各治疗组连续8周颈背部皮下注射D-半乳糖溶液，同时进行相应的治疗干预。造模结束后检测各组小鼠血液衰老相关T淋巴细胞百分比、血清白细胞介素2、白细胞介素4水平并观察脾脏、胸腺组织结构。结果与结论：3个治疗组均能提高衰老小鼠血液CD3+、CD8+CD28+T细胞百分比及血清白细胞介素2、白细胞介素4水平(P 0.01)；与模型组相比，联合组初始性CD8+T细胞百分比升高，记忆性CD8+T细胞百分比降低(P 0.05)，干细胞及番茄红素组初始性及记忆性CD8+T细胞百分比变化不显著(P 0.05)；各组初始性及记忆性CD4+T细胞百分比变化均不显著(P 0.05)；胸腺、脾脏镜下观察发现：模型组胸腺小体缩小，脾脏淋巴小结明显增多，髓质淤血；正常对照组与3个治疗组胸腺、脾脏皮质髓质均未见明显病变。结果表明人脐带间充质干细胞、番茄红素均可以不同程度改善衰老小鼠免疫功能，且联合治疗效果更优。 中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程 全文链接： 关键词?：?干细胞；?脐带脐血干细胞；?人脐带间充质干细胞；?番茄红素；?初始性及记忆性T细胞；?CD8+CD28+T细胞； ?? Abstract： BACKGROUND: Both human umbilical cord mesenchymal stem cells and lycopene have the anti-aging actions. But the research about their combination effects on immune function of aging body has not been reported.OBJECTIVE: To investigate the effects of human umbilical cord mesenchymal stem cells combined with lycopene on immune function of D-galactose induced aging miceMETHODS: ICR mice, female and male in half, were randomly divided into five groups: normal control group, model group, lycopene group, stem cell group, stem cell combined with lycopene group (combined group). Model group and treatment groups were injected subcutaneously with D-galactose at the neck for 8 weeks. Age-related pe