家蚕触角富集UDP葡萄糖醛酸基转移酶基因的克隆和表达分析_英文_.pdfVIP

Science of Sericulture 蚕业科学 2011，37 (3):0406 － 04 11 ISSN 0257 － 4799 ;CN 32 － 1115 / S E-mail :CYKE@ chinajournal． net． cn Cloning and Expression Analysis of an Antennal-enriched UDP- glucosyltransferase Gene from Silkworm ，Bombyx mori * HUANG Fei-Fei ZHANG Jun HUANG Mao-Hua WANG Ting-Ting PENG Li-Na PAN Min-Hui * LU Cheng (Institute of Sericulture and Systems Biology ，Southwest University ，Chongqing 4007 16 ，China) Abstract Glucosidation plays a major role in the inactivation and excretion of a wide variety of endogenous and exogenous compounds． This process involves a class of UDP-glycosyltransferases (UGTs)． We report here the molecular cloning and expression characteriza- tion of the silkworm antennal-enriched UGT BmUGT013829 ． We cloned a complete 1 545 bp cDNA that encodes a 514 amino acid pro- tein． Western blotting revealed that BmUGT013829 was expressed in the fat body ，head ，integument and midgut ，and the protein was lowly expressed in the midgut and highly expressed in head． Immunohistochemical assay found that BmUGT013829 expression level was the highest in antennae compared to other components of the head． These patterns suggest that BmUGT013829 is an antennal-enriched UGT and may play a role in olfaction． Key words Bombyx mori ;Antennal enrichment ;UDP-glucosyltransferase ;Tissue expression 家蚕触角富集 UDP 葡萄糖醛酸基转移酶基因的克隆与表达 分析 黄飞飞 张 军 黄茂华 王婷婷 彭丽娜 潘敏慧 鲁 成 ( ， 4007 16) 西南大学蚕学与系统生物学研究所 重庆 ， - (UDP- 摘 要 糖基化作用在抑制和排除一系列内生和外源化合物的毒性方面起重要作用 一系列尿苷二磷酸糖基转移酶 glycosyltransferases ，UGTs) 。 UDP