《Ni柱亲和层析》.pdf

FF 6% 50mol /ml 45mg/ml 45-165 m 300cm/h pH 3-10pH 2-11 +4~8 20% His FF His 1 FF 1.6×20m10ml 21 2-5 2ml/min 320ml (50 mM PBSpH7.40.5M NaCl)0.45 m 1ml/min 41 2-5 2ml/min 59 409 100036 TE FAX 5102050100200300400mM mM 3 2ml/minSDS 65 20%3 2ml/min +4~8 71SDS 150mM pH7.4 PBS0.5M NaH PO 19ml0.5M Na HPO 81ml 2 4 2 4 NaCl 29.3g, 1000ml 2 50mMpH7.4pH7.4 PBS0.5M NaH PO 19ml 2 4 0.5M Na HPO 81mlNaCl 29.3g34g, 1000ml 2 4 3 B 1 (ml) 2 (ml) 10 mM 98 2 20 mM 96 4 50 mM 90 10 100 mM 80 20 200 mM 60 40 300mM 40 60 400 mM 20 80 SDS 1 BCA 2 5-10µg/ 3 1.5ml EP 5-10µg 10µl20mM PBS pH7.4 10µl10µl1