《亲和层析柱说明书》.pdf

® 生工 生物工程（上海）有限公司 GST SefinoseTM Resin Product information forBSP032-7/BSP031/BSP031-1/BSP091-1/BSP091-3： Introduction The glutathione ligand is coupled via a 10-carbon linker to highly cross-linked 4% agarose. The coupling of Glutathione Sefinose™ resin is optimized to provide high binding capacity and one-step purification of glutathione S-transferase (GST) tagged proteins expressed from pGEX series vectors, as well as other glutathione S-transferases and glutathione binding proteins. GST-tagged proteins can be purified directly from pre-treated bacterial lysates using Glutathione Sefinose resin. The tagged proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity and function. The Glutathione sefinose resin is an excellent choice for high performance purifications. Glutathione sefinose resin is also available in conveniently pre-packed 1ml, 5ml gravity flow column . If removal of the GST moiety (a naturally occurring protein with Mr 26 000) is required, the tagged protein can be digested with appropriate site-specific protease while bound to Glutathione Sefinose or, alternatively after elution. Cleavage of GST-tagged protein bound to resin eliminates the extra step of separating the released protein from GST. The cleaved target protein is eluted using binding buffer. Features Average bead size 50 μm Protein binding capacity* Approx.5-10 mg GST-tagged protein/1ml resin Max flow velocity 300cm/h. Ligand density 10-20umol/ml GST resin. pH stability, short-term (2 h) 3–12 Storage 20% ethanol Storage temperature +4 to +30ºC Notice: Binding of GST to glutathione is flow-rate dependent. The lower the flow rate, the higher the binding capacity. Flow rate is also essential during sample loading and elution steps Recommended Buffers Binding/wash buf