《Near and Mid Infrared Spectroscopy to Detect Malolactic Biotransformation of Oenococcus oeni in a Wine-Model》.pdf

JournalofAgriculturalScienceandTechnologyA4(2014)475-486 Earliertitle：JournalofAgriculturalScienceandTechnology，ISSN 1939—1250 … ～ … NearandMidInfraredSpectroScopytoDetectMalolactic B一I●otransf一ormati。onof一0Aenococcusoenl●i■naW●-■I●ne·M-IOa■eI● VigentiniIleana，GrassiSilvia，SinelliNicoletta，DiEgidioValentina，PicozziClaudia，FoschinoRoberto and CasiraghiErnestina DepartmentofFood,EnvironmentalandNutritionalSciences(DEFENS)，Milan20133，~taly Received：November6，2013／Published：June20，2014 Abstract：Theaim ofthisworkwastoverifythepotentialofinfrared(IR)spectroscopyinnearandmidregionstodetectthe beginningofthemalolacticfermentation(MLF)occurringinamodel—wineandthefurthercellsautolysis．MLF inwineisa secondarybiotransformationduetolacticacidbacteriathatusuallyoccursspontaneouslyorafterstarterinoculationattheendof alcoholicfermentation．Nowadays．itisdesirabletosupplywinemakerswithanew rapidandnon-destructiveapproachtomonitor M LF progressand 1R spectroscopytechnologyappearstobesuitableforthispurpos．Thetransfomr ation ofL—malicacid into L—lacticacid wascarriedoutby inoculating asynthetic winewithan Oenococcusoenicultureand itwasmonitored through microbiologicalandchemicalmethods．Atthesametime，Fouriertransofrm nearinfrared (FTN·IR)specialdata，indiffusive transflectionmodeusinganopticprobe，andFT．IRspectra，usingangermaniumcrystalattenuatedtotalreflectance(ATR)cell，were collected．Principalcomponentanalysisofthe spectrawasable to identi absorptionbna dsrelated to thekey molecular modificationsthattook place duringthe L—malicacid transformation．Thus，the sampleswerediscriminatedaccording to the fermentationphase．Althoug【hthisstudyisapreliminaryapproach，resultsconfirmthatnearinfrared(NIR)andmidinrfared(MIR) spectroscopycouldbesuccessfullyappliedtodetectthestartofMLFandtheautolysisofthelacticacidbacteria(LAB)cells． Keywords：Malolacticfemr entation，wine，FT-IR