《Over-expression of Testis-specific Expressed Gene 1 Attenuates the Proliferation and Induces Apoptosis of GC-1spg Cells》.pdf

JHuazhongUnivSciTechnol[MedSci] 34(4)：535—5412014 ， DOIlO．1007／sl1596一O14．13l1-3 535 Over．．expression ofTestis．．specificExpressed Gene 1Attenuatesthe ProliferationandInducesApoptosisofGC．1spgCells。 Chap．huiGU(顾朝辉)，，，Feng．yanTIAN(田凤艳)”，Jia．ruiPU(普嘉瑞)，一，Li．duanZHENG(郑丽端)，HongMEI(梅 红)一， Fu．qingZENG(曾甫清)，Jin-jianYANG(杨锦建)，Quan．chengKAN (阚全程)，Qiang．songTONG(童强松)，， FirstAffiliatedHospitalZhengzhouUniversity,Zhengzhou450052，China 2DepartmentofSurgery,3HumanDiseaseRelatedGeneResearchGroup，4DepartmentofPathology,UnionHospitalTongjiMedical College,HuazhongUniversiytofScienceandTechnology,Wuhan430022,China ‘ ◎HuazhongUniversityofScienceandTechnologyandSpringer-VerlagBerlinHeidelberg2014 Summary：Theeffectsofover．expressionoftestis—specificexpressedgene1(TSEG．1、ontheviability and apoptosisofcultured spermatogonialGC．1spgcellswereinvestigated．and theimmorta1sper． matogonia1celllineGC一1spg(CRL．2053MT)wasobtainedasthecellmodelinordertoexplorethe function0fTSEG．1．W etransfectedtheeukaryoticvectorOfTSEG．1．namedaspEGFP—TSEG．1into cultured spermatogonia1GC．1sDg cells．Over．expression ofTSEG一1inhibited the proliferation of GC．1spgcells，andarrestedeellcycleslightlyatGn，G1phase．TransfectionofTSEG．1attenuatedthe transcriptlevelsofKi．67．PCNA andcyclinD1．Inaddition．over-expressionofTSEG．1inducedearly and lateapoptosis．andreduced themitochondrialmembranepotentialofGC．1spgcells．Moreover， rtansfectionOfTSEG．1significnatlyenhancedtheratioofBax／Bcl一2andrtanscriptlevelsofcaspase9． and decreased the expression ofFas na d cast