《Relationships Between C4 Enzyme Activities and Yield in Soybeans （Glycine max （L.） Merr.）》.pdf

Availableonlineatwww．sciencedirect．com RelationshipsBetweenC4EnzymeActivitiesandYieldinSoybeans( max(L．)Merr．) HUANGShan—shan1，LIChang—SUO，，YANGMing—liang，LIWen—binandWANGJi-an KeyLaboratoryofSoybeanBiology，Ministryof Education~SoybeanResearchInstitute，NortheastAgriculturalUniversity，Harbin 150030，P．R．China PlantProtectionInstitute，HeilongjiangAcademyofLandReclamationSciences，Harbin150038，P．R．China TostudytherelationshipsbetweenC4enzymeactivitiesandyield，C4enzymeactivities(phosphoenolpyruvatecarboxylase (PEPCase)，NADP—malatedehydrogenase(NADP—MDH)，NADP—malicenzyme(NADP-ME)，andpyruvatephosphate dikinase(PPDK))indifferentorgansoftensoybeancultivarswithdifferentyieldsweremeasuredatdifferentgrowth stagesinChina．TheresultshowedthatfourenzymeactivitiesinC4pathwaywereobviouslydifferentamongcultivars， especiallyPPDKactivitywasnotdetectedintheleavesofDongnong1567andDongnong 1068andtheyoungleavesof Gongjiao9107—1andDongnong97-172，buttherewereweakactivitiesinpodcoats．TheorderofC4enzymeactivitiesis youngleavesold leavespod coats．ThecorrelationcoefficientsbetweenPEPCaseactivityandyieldandbetween NADP—MDH activityatbloomingstageandyieldwere0．6979and0．6565，respectively，andbothreachedthesignificant level(5％)，andPEPCaseactivitykeptsignificantpositivecorrelationwithplantphotosyntheticrate．Therewasanegative correlationbetweenNADP-MEactivityandyield．andnocorrelationwasfoundbetweenPPDK activityandyield． Keywords：soybean，photosyntheticrate，yield，C4enzyme CO deficiency(Cedingeta1．1997)．Inthecaseof INTRODUCTlON lowerC0 concentration，throughtheenzymesystem： PEPCase，NADP—M DH，NADP．ME，andPPDK，C0， Landplantscanbedividedlntothreemajorphotosyn— iseffectivelyassimilated，andC4pathwaymakescrops thetictypes：C3，C4，andcrassulaceanacidmetabolism