Double-stranded RNA transcribed from vector-based oligodeoxynucleotide acts as transcription factor decoy》.pdf

Biochemical and Biophysical Research Communications 457 (2015) 221–226 Contents lists available at ScienceDirect Biochemical and Biophysical Research Communications journal homepage: www.else /locate/ybbrc Double-stranded RNA transcribed from vector-based oligodeoxynucleotide acts as transcription factor decoy Xiao Xiao a,1, Yi Gang a,b,1, Honghong Wang c, Jiayin Wang d, Lina Zhao e, Li Xu a,⇑, Zhiguo Liu a,⇑ a State Key Laboratory of Cancer Biology and Xijing Hospital of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, PR China b Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, Shaanxi Province, PR China c No. 518 Hospital of Chinese People’s Liberation Army, Xi’an 710043, Shaanxi Province, PR China d The Genome Institute, Washington University in St. Louis, St. Louis, MO 63108, USA e Department of Radiation Oncology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, PR China a r t i c l e i n f o a b s t r a c t Article history: In this study, we designed a short hairpin RNA vector-based oligodeoxynucleotide (VB-ODN) carrying Received 2 December 2014 transcription factor (TF) consensus sequence which could function as a decoy to block TF activity. Specif- Available online 27 December 2014 ically, VB-ODN for Nuclear factor-jB (NF-jB) could inhibit cell viability and decrease downstream gene expression in HEK293 cells without affecting expression of NF-jB itself. The specific binding between VB- Keywords: