Effect of doping pretreated corn stover conditions on yield of bioethanol in immobilized cell systems》.pdf

Renewable Energy 86 (2016) 858e865 Contents lists available at ScienceDirect Renewable Energy journal homepage: /locate/renene Effect of doping pretreated corn stover conditions on yield of bioethanol in immobilized cell systems Shuang-Qi Tian a, b, *, Xin-Wei Wang a, b, Ren-Yong Zhao a, Sen Ma a, b a College of Food Science and Technology, Henan University of Technology, Zhengzhou 450001, China b National Engineering Laboratory for Wheat and Corn Further Processing, Zhengzhou 450001, China a r t i c l e i n f o a b s t r a c t Article history: The surface characteristics of immobilized yeast before and after adding CO -laser pretreated corn stover 2 Received 10 March 2015 (LPCS) substrates were investigated using bioethanol production. Response surface methodology (RSM), Received in revised form based on the BoxeBehnken design (BBD) for experiments, was used to optimize the doping condition. An 10 August 2015 optimum experimental condition was obtained at pH 4.5, 2.08% yeast concentration, and 0.20% LPCS Accepted 10 September 2015 Available online xxx substrates. Under this condition, doping LPCS increased the yield of bioethanol from 53% to 84%, which matched the predicted value. After doping LPCS, the results of inverted microscope (IM) and atomic force