Effect of proline supplementation on anti-oxidative capacity, immune response and stress tolerance of juvenile Pacific white shrimp, Litopenaeus vannamei》.pdf

Aquaculture 448 (2015) 105–111 Contents lists available at ScienceDirect Aquaculture journal homepage: www. elsevi er. com/ locate /aqua-online Effect of proline supplementation on anti-oxidative capacity, immune response and stress tolerance of juvenile Pacific white shrimp, Litopenaeus vannamei Shi-Wei Xie, Li-Xia Tian, Yu-Ming Li, Weiwen Zhou, Shuai-Lin Zeng, Hui-Jun Yang, Yong-Jian Liu ⁎ Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou, China a r t i c l e i n f o a b s t r a c t Article history: An 8-week feeding trial was conducted to evaluate the effect of proline on anti-oxidative capacity, immune re- Received 1 April 2015 sponse and stress tolerance of juvenile Pacific white shrimp, Litopenaeus vannamei. Six practical low fishmeal Received in revised form 22 May 2015 diets (15% fishmeal) were formulated to contain graded levels (2.02, 2.14, 2.27, 2.38, 2.49, 2.6%) of L-proline. Accepted 23 May 2015 Each diet was randomly assigned to triplicate groups of 30 shrimps (approximately 0.41 g) and the shrimps Available online 29 May 2015 were fed 4 times a day to apparent satiation. Growth performance was not affected by proline levels Keywords: (P N 0.05). But higher levels of proline increased hydroxyproline content in the whole body (P = 0.004). And sur- Litopenaeus vannamei vival after 60 h acute NH3 stress increased with the increasing dietary proline levels (P =