Effects of hydroxysafflor yellow A on the activity and mRNA expression of four CYP isozymes in rats》.pdf

Journal of Ethnopharmacology 151 (2014) 1141 –1146 Contents lists available at ScienceDirect Journal of Ethnopharmacology journal homepage: /locate/jep Effects of hydroxysafflor yellow A on the activity and mRNA expression of four CYP isozymes in rats Ren-ai Xu a,b, Zhi-sheng Xu b, Ren-shan Ge a,b,n a The First Affi liated Hospital of Wenzhou Medical University, Wenzhou 325000, China b The Second Affi liated Hospital of Wenzhou Medical University, Wenzhou 325000, China a r t i c l e i n f o a b s t r a c t Article history: Ethnopharmacological relevance: In traditional therapy with Chinese medicine, hydroxysafflor yellow A Received 10 July 2013 (HSYA), a main active component isolated from the dried flower of Carthamus tinctorius L., is the principal Received in revised form efficiency ingredient of Safflor Yellow Injection. Now HSYA has been demonstrated to have good 14 December 2013 pharmacological activities of antioxidation, myocardial and cerebral protective and neuroprotective Accepted 18 December 2013 Available online 27 December 2013 effects. The purpose of this study was to find out whether HSYA influences the effect on rat cytochrome P450 (CYP) enzymes (CYP1A2, CYP2C11, CYP2D4 and CYP3A1) by using cocktail probe drugs in vivo; the Keywords: influence on the levels of CYP mRNA was also studied. Hydroxysafflor yellow A Materials and methods: A cocktail solution at a dose of 5 mL/kg, which contained ph