Efficient expression, purification and characterization of native human cystatin C in Escherichia coli periplasm》.pdf
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Efficient expression, purification and characterization of native human cystatin C in Escherichia coli periplasm》.pdf
Protein Expression and Purification 111 (2015) 18–22
Contents lists available at ScienceDirect
Protein Expression and Purification
journal homepage: /locate/yprep
Efficient expression, purification and characterization of native human
cystatin C in Escherichia coli periplasm
Yongjun Zhou a, Yan Zhou b, Jun Li a, Jian Chen a, Yuqin Yao a, Lin Yu a, Desheng Peng c, Mingrong Wang c,
Dan Su a, Yong He a,⇑, Lantu Gou a,⇑
a State Key Laboratory of Biotherapy/Collaborative Innovation Center for Biotherapy, West China Medical School, West China Hospital, Sichuan University, Chengdu, PR China
b The Gastroenterology Tumor and Microenvironment Laboratory, Department of Gastroenterology, The First Affiliated Hospital of Chengdu Medical College, Chengdu Medical
College, Chengdu, PR China
c Chengdu Institute of Biological Products Co., Ltd., PR China
a r t i c l e i n f o a b s t r a c t
Article history: Human cystatin C (HCC), encoded by cystatin 3 gene, is a 13.3 kDa endogenous cysteine proteinase
Received 3 February 2015 inhibitor and an important biomarker of renal function. However, expressing recombinant cystatin C is
and in revised form 11 March 2015 difficult because of low yield and inclusion bodies in Escherichia coli (E. coli). In this study, we cloned
Available online 17 March 2015
HCC gene into pET-22b vector containing PelB leader signal sequence, which could direct the protein to
the bacterial periplasm. Large amounts of soluble HCC could be efficiently expressed
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