Electroimmunoassay of human serum lipoproteins containing Apo A-I by monoclonal antibodies》.pdf

Clinica Chimica Acta, 172 (1988) 149-160 149 Elsevier CCA 04081 Electroimmunoassay of human serum lipoproteins containing Apo A-I by monoclonal antibodies M. Ayrault-Jarrier a, E. Petit a, E.D. Bekaert a, H. Robin a, D. Pastier a, I. Beucler a and J. Polonovski a, E. Hervaud b and B. Pau b a CNRS- UA 524, Laboratoire de Biochimie, UFR Saint-Antoine, Paris and ’ Centre de Recherche Clin-Midy (Sanofi), Montpellier (France) (Received 27 July 1987; revision received 1 September 1987; accepted after revision 1 November 1987) Key words: Electroimmunoassay; Lipoproteins; Apolipoprotein A-I; Monoclonal antibody; Human serum Summary Three monoclonal antibodies to human serum apolipoprotein (APO) A-I (4A12, 4Bll and 2Gll) were produced by Sanofi. They were directed to three distinct epitopes of the Apo A-I molecule, present on the surface of the lipoprotein particles. They were able to precipitate individually some lipoprotein units from the serum. Only the mixture of the three monoclonal antibodies could allow a precipitation of all Apo A-I containing particles. An electroimmunoassay using this oligoclonal mixture was assessed to standardize the Apo A-I measurement in serum. Results agreed well with those obtained