Enzymatic hydrolysis of polyethylene terephthalate films in an ultrafiltration membrane reactor》.pdf

Journal of Membrane Science 494 (2015) 182 –187 Contents lists available at ScienceDirect Journal of Membrane Science journal homepage: /locate/memsci Enzymatic hydrolysis of polyethylene terephthalate films in an ultrafiltration membrane reactor Markus Barth, Ren Wei, Thorsten Oeser, Johannes Then, Juliane Schmidt, Franziska Wohlgemuth, Wolfgang Zimmermann n Department of Microbiology and Bioprocess Technology, Institute of Biochemistry, University of Leipzig, Johannisallee 21-23, D-04103 Leipzig, Germany a r t i c l e i n f o a b s t r a c t Article history: Synthetic aromatic polyesters such as polyethylene terephthalate (PET) are degraded by microbial Received 1 May 2015 polyester hydrolases at mild reaction conditions compared to chemical processes. PET films were hy- Received in revised form drolyzed by the polyester hydrolase TfCut2 from Thermobifi da fusca KW3 in an aqueous reaction system 13 July 2015 contained in an ultrafiltration membrane reactor. The continuous removal of inhibitory hydrolysis pro- Accepted 14 July 2015 ducts by the ultrafiltration membrane increased the efficiency of the biocatalytic hydrolysis of PET by 70% Available online 21 July 2015 over a reaction time of 24 h compared to a hydrolysis performed in batch mode. A model describing the Keywords: biocatalytic hydrolysis of PET films with simultaneous ultrafiltration to remove the reaction products w